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pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
(Plasmid #52689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-kanMX-tetO-Pcyc1-3xFLAG (41022)
  • Vector type
    Yeast Expression ; Promoter replacement
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tetO7-Pcyc1-3xFLAG+LINKER
  • Species
    S. pombe (fission yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is the same as the "regular" tetO plasmids (41022, 41023, 41024, 41025, or 41026) with an additional LADYGAG linker. This allows amplification of tagging cassettes from the LINKER vectors using the same pair of primers. For the “regular” vectors the reverse primer is unique to each plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER was a gift from Michael Nick Boddy (Addgene plasmid # 52689 ; http://n2t.net/addgene:52689 ; RRID:Addgene_52689)
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