pcDNA3-Abl-His6-FLAG
(Plasmid #52684)

• Purpose: expression of Abl in mammalian cells
• Depositing Lab: Benjamin Turk
• Publication: Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2. doi: 10.1002/cbic.201200560. Epub 2012 Oct 18.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABL1
• Alt name: c-Abl
• Alt name: c-abl oncogene 1, non-receptor tyrosine kinase
• Species: M. musculus (mouse)
• Insert Size (bp): 3426
• GenBank ID: 11350
• Entrez Gene: Abl1 (a.k.a. Abl, E430008G22Rik, c-Abl)
• Promoter: CMV
• Tags / Fusion Proteins:
  • His6 (C terminal on backbone)
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tony Koleske
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes isoform IV of mouse c-Abl, which is N-myristylated.