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pGEX4T2-Snf1-cat
(Plasmid #52683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNF1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    822
  • Mutation
    encodes kinase domain only, aa 41-315
  • GenBank ID
    852088
  • Entrez Gene
    SNF1 (a.k.a. YDR477W, CAT1, CCR1, GLC2, HAF3, PAS14)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T2-Snf1-cat was a gift from Benjamin Turk (Addgene plasmid # 52683 ; http://n2t.net/addgene:52683 ; RRID:Addgene_52683)

  • For your References section:

    Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress. Lee YJ, Jeschke GR, Roelants FM, Thorner J, Turk BE. Mol Cell Biol. 2012 Nov;32(22):4705-17. doi: 10.1128/MCB.00897-12. Epub 2012 Sep 17. 10.1128/MCB.00897-12 PubMed 22988299
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