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Purpose(Empty Backbone) shRNA silencing plasmid with eGFP transfection marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSILENCER
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Backbone manufacturerLife Technologies
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Modifications to backboneeGFP and its cytomegalovirus promoter from pEGFP-C3 were cloned into the KpnI site upstream of the U6 promoter within the pSilencer™ vector.
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Vector typeRNAi
- Promoter CMV and U6
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Tag
/ Fusion Protein
- CMV-EGFP (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EGFP is a transfection marker, not a fusion.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIL-eGFP was a gift from Johannes Hell (Addgene plasmid # 52675 ; http://n2t.net/addgene:52675 ; RRID:Addgene_52675) -
For your References section:
The cytoskeletal protein alpha-actinin regulates acid-sensing ion channel 1a through a C-terminal interaction. Schnizler MK, Schnizler K, Zha XM, Hall DD, Wemmie JA, Hell JW, Welsh MJ. J Biol Chem. 2009 Jan 30;284(5):2697-705. doi: 10.1074/jbc.M805110200. Epub 2008 Nov 21. 10.1074/jbc.M805110200 PubMed 19028690
Map uploaded by the depositor.
