PSD-95-pTagRFP
(Plasmid #52671)

• Purpose: expression of TagRFP-tagged PSD-95
• Depositing Lab: Johannes Hell
• Publication: Hell lab plasmids (unpublished)

Backbone

• Vector backbone: pTagRFP
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSD-95
• Alt name: Postsynaptic density protein 95
• Alt name: Disks large homolog 4 (DLG4)
• Alt name: Synapse-associated protein 90 (SAP-90)
• Species: R. norvegicus (rat)
• GenBank ID: P78352
• Entrez Gene: Dlg4 (a.k.a. Dlgh4, PSD95, Sap90)
• Promoter: CMV
• Tag / Fusion Protein:
  • tagRFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: mKate2-TurboRFP-R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HindIII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N.