Fast-GCaMP-RS09
(Plasmid
#52649)
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Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activity
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerMillipore
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6722
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Modifications to backboneinserted Fast GCaMP RS09 between NotI and XbaI
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFast-GCaMP-RS09
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1353
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MutationL414T mutated from GCaMP3
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer 5'-d(TAA TAC GAC TCA CTA TAG GG)-3'
- 3′ sequencing primer 5'-d(GCT AGT TAT TGC TCA GCG G)-3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMillipore (catalog number 69865)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fast-GCaMP-RS09 was a gift from Samuel Wang (Addgene plasmid # 52649 ; http://n2t.net/addgene:52649 ; RRID:Addgene_52649) -
For your References section:
Fast GCaMPs for improved tracking of neuronal activity. Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, Hogue IB, Enquist LW, Murthy M, Wang SS. Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170. 10.1038/ncomms3170 PubMed 23863808