Fast-GCaMP-EF20
(Plasmid #52645)

• Purpose: genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
• Depositing Lab: Samuel Wang
• Publication: Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Millipore
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6722
• Modifications to backbone: inserted Fast GCaMP EF20 between NotI and XbaI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fast-GCaMP-EF20
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1353
• Mutation: D362N/D366N mutated from GCaMP3
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: 5'-d(TAA TAC GAC TCA CTA TAG GG)-3'
• 3′ sequencing primer: 5'-d(GCT AGT TAT TGC TCA GCG G)-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Millipore (catalog number 69865)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry