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Addgene

Fast-GCaMP-EF20
(Plasmid #52645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Millipore
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6722
  • Modifications to backbone
    inserted Fast GCaMP EF20 between NotI and XbaI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fast-GCaMP-EF20
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1353
  • Mutation
    D362N/D366N mutated from GCaMP3
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-d(TAA TAC GAC TCA CTA TAG GG)-3'
  • 3′ sequencing primer 5'-d(GCT AGT TAT TGC TCA GCG G)-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Millipore (catalog number 69865)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fast-GCaMP-EF20 was a gift from Samuel Wang (Addgene plasmid # 52645 ; http://n2t.net/addgene:52645 ; RRID:Addgene_52645)

  • For your References section:

    Fast GCaMPs for improved tracking of neuronal activity. Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, Hogue IB, Enquist LW, Murthy M, Wang SS. Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170. 10.1038/ncomms3170 PubMed 23863808
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