p(HA)HIF1alpha (P402A,P564A)
(Plasmid #52636)

• Purpose: mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation
• Depositing Lab: Eric Huang
• Publication: Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 2004 Apr 14.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIF1alpha P402A, P564A
• Alt name: HIF1A
• Alt name: hypoxia inducible factor 1, alpha subunit
• Species: H. sapiens (human)
• Mutation: P402A, P564A
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry