Skip to main content
Addgene

pLVE-eGFP
(Plasmid #52581)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52581 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLVU-tTR-KRAB
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 9969
  • Modifications to backbone
    Exchanged UbC promoter and eGFP-IRES-tTRKRAB insert for EF-1alpha promoter + MCR and later cloned in an eGFP insert. The lentiviral cPPT is now located between the promoter and the insert.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720
  • Promoter EF-1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer CCA GCT TGG CAC TTG ATG TA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVE-eGFP was a gift from Charles Gersbach (Addgene plasmid # 52581 ; http://n2t.net/addgene:52581 ; RRID:Addgene_52581)
  • For your References section:

    Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, Sanchez-Adams J, Gersbach CA, Guilak F. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18. 10.1073/pnas.1321744111 PubMed 24550481