pLVE-eGFP
(Plasmid #52581)

• Purpose: Lentiviral transfer vector driving expression of eGFP in mammalian cells
• Depositing Lab: Charles Gersbach
• Publication: Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18.

Backbone

• Vector backbone: pLVU-tTR-KRAB
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 9969
• Modifications to backbone: Exchanged UbC promoter and eGFP-IRES-tTRKRAB insert for EF-1alpha promoter + MCR and later cloned in an eGFP insert. The lentiviral cPPT is now located between the promoter and the insert.
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 720
• Promoter: EF-1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NheI (destroyed during cloning)
• 5′ sequencing primer: CCA GCT TGG CAC TTG ATG TA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry