pLVE-hTGFB3-IRES-Red
(Plasmid #52580)

• Purpose: 2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells
• Depositing Lab: Charles Gersbach
• Publication: Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18.

Backbone

• Vector backbone: pLV-tTRKRAB-red
• Backbone size w/o insert (bp): 10527
• Total vector size (bp): 12293
• Modifications to backbone: Added MCS downstream of EF-1alpha promoter in backbone prior to cloning.
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human transforming growth factor-beta3
• Alt name: TGF-beta3
• Species: H. sapiens (human)
• Insert Size (bp): 1239
• GenBank ID: NM_003239.2
• Entrez Gene: TGFB3 (a.k.a. ARVD, ARVD1, LDS5, RNHF, TGF-beta3)
• Promoter: EF-1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: CCA GCT TGG CAC TTG ATG TA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry