pRB20
(Plasmid
#52559)
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Purposeserves as PCR-template to generate HR donors for C-terminal YFP-tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJet1.2
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Backbone manufacturerThermor Fisher / Fermentas
- Backbone size w/o insert (bp) 2972
- Total vector size (bp) 4477
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Vector typeblunt cloning kit
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeYFP and Blasticigin resistance cassette
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SpeciesSynthetic
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Insert Size (bp)720
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer taatacgactcactataggg (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB20 was a gift from Klaus Foerstemann (Addgene plasmid # 52559 ; http://n2t.net/addgene:52559 ; RRID:Addgene_52559) -
For your References section:
Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K. Nucleic Acids Res. 2014 Apr 19. 10.1093/nar/gku289 PubMed 24748663
Map uploaded by the depositor.
