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Addgene

Fast-GCaMP-EF05
(Plasmid #52558)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJFRC-MUH
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 7831
  • Total vector size (bp) 9184
  • Modifications to backbone
    insert between BglII and NotI
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fast-GCaMP-EF05
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1353
  • Mutation
    changed from GCaMP3: D397N/G398A/N399D

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TACT AGATCT GCCGCCACC atgggttctcatcatcat
  • 3′ sequencing primer att cca cca ctg ctc cca
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene 26213

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fast-GCaMP-EF05 was a gift from Samuel Wang (Addgene plasmid # 52558 ; http://n2t.net/addgene:52558 ; RRID:Addgene_52558)

  • For your References section:

    Fast GCaMPs for improved tracking of neuronal activity. Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, Hogue IB, Enquist LW, Murthy M, Wang SS. Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170. 10.1038/ncomms3170 PubMed 23863808
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