pBT344_pattB-pCA-GFP-pA-FRT5
(Plasmid #52550)

• Purpose: for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice
• Depositing Lab: Liqun Luo
• Publication: Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 10.1073/pnas.1019507108. Epub 2011 Apr 4.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PhiC31o attB (full length)

Gene/Insert 2

• Gene/Insert name: GFP

Resource Information

• Supplemental Documents:
  • pBT344_Genbank_file
• A portion of this plasmid was derived from a plasmid made by: Full length attB from Michele Calos

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry