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pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
(Plasmid #52542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PhiC31o attPx3 (70 bp each)

Gene/Insert 2

  • Gene/Insert name
    Neo

Gene/Insert 3

  • Gene/Insert name
    PhiC31o integrase

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PhiC31o from Phil Soriano

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 was a gift from Liqun Luo (Addgene plasmid # 52542 ; http://n2t.net/addgene:52542 ; RRID:Addgene_52542)

  • For your References section:

    Site-specific integrase-mediated transgenesis in mice via pronuclear injection. Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 10.1073/pnas.1019507108. Epub 2011 Apr 4. 10.1073/pnas.1019507108 PubMed 21464299
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