RGS-6xHis-BLIMP-1-pcDNA3.1-
(Plasmid #52518)

• Purpose: expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus
• Depositing Lab: Adam Antebi
• Publication: Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028.

Backbone

• Vector backbone: pcDNA3.1-
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 7899
• Modifications to backbone: addded/inserted sequence encoding "RGS-6xHis" tag at start of MCS to allow for fusion protein production
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Blimp1
• Alt name: PRDM1
• Species: H. sapiens (human)
• Insert Size (bp): 2484
• GenBank ID: EU831954.1 ace87395
• Entrez Gene: PRDM1 (a.k.a. BLIMP1, PRDI-BF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert cDNA was amplified from DF/HCC PlasmID Cat# HsCD00296049
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry