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RGS-6xHis-LIN-29-pcDNA3.1-
(Plasmid #52515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5442
  • Total vector size (bp) 6817
  • Modifications to backbone
    addded/inserted sequence encoding "RGS-6xHis" tag at start of MCS to allow for fusion protein production
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lin-29 b
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1375
  • Mutation
    wt (changed coding sequence in 5' (5th codon from GTT to GTA) to mutate internal Xba I site, coding of sequence was conserved)
  • Entrez Gene
    lin-29 (a.k.a. CELE_W03C9.4)
  • Promoter CMV
  • Tag / Fusion Protein
    • RGS-6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-polyA reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert cDNA was amplified from Open Biosystems Cat# OCE4818-99849527 (W03C9.4 lin-29 C. elegans Transcription Factor Clone - pDESTAD-amp)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RGS-6xHis-LIN-29-pcDNA3.1- was a gift from Adam Antebi (Addgene plasmid # 52515 ; http://n2t.net/addgene:52515 ; RRID:Addgene_52515)

  • For your References section:

    DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028. 10.1016/j.devcel.2014.01.028 PubMed 24613396
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