FLAG-HA-BLMP-1-pcDNA3.1-
(Plasmid #52512)

• Purpose: expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus
• Depositing Lab: Adam Antebi
• Publication: Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028.

Backbone

• Vector backbone: pcDNA3.1-
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5462
• Total vector size (bp): 7922
• Modifications to backbone: added FLAG-HA tag in front of MCS, tag coding sequence has: Kozak – FLAG - 5x Glycine – HA – 5x Glycine – MCS ...
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Blmp-1
• Species: C. elegans (nematode)
• Insert Size (bp): 2460
• Mutation: N75S mutation compared GenBank reference sequence NP_001251370.1
• Entrez Gene: blmp-1 (a.k.a. CELE_F25D7.3)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert cDNA was amplified from Open Biosystems Cat# OCE1182-7241376 (F25D7.3 C. elegans ORF Clone Caenorhabditis elegans pDONR201)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry