FLAG-HA-mRFP-pcDNA3.1-
(Plasmid #52510)

• Purpose: expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus
• Depositing Lab: Adam Antebi
• Publication: Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028.

Backbone

• Vector backbone: pcDNA3.1-
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5439
• Total vector size (bp): 6123
• Modifications to backbone: added FLAG-HA tag in front of MCS, tag coding sequence has: Kozak – FLAG - 5x Glycine – HA – 5x Glycine – MCS ...
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRFP
• Species: discosoma
• Insert Size (bp): 684
• Mutation: engineered variant of tetrameric red fluorescent protein from Discosoma GenBank Accession Number AAF03369
• GenBank ID: AF506027.1
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: original cDNA for mRFP generated by R.Y. Tsien lab: Campbell, R.E., Tour, O., Palmer, A.E., Steinbach, P., Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 2002. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. 99: 7877-7882. Accession: AF506027

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry