pcDNA5-FRT/T0-Flag-53BP1
(Plasmid #52507)

• Purpose: mammalian expression vector of 53BP1
• Depositing Lab: Daniel Durocher
• Publication: Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.

Backbone

• Vector backbone: pcDNA5 FRT/T0-Flag
• Backbone size w/o insert (bp): 5198
• Total vector size (bp): 11114
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1
• Species: H. sapiens (human)
• Insert Size (bp): 5916
• Mutation: V128A and siRNA resistant sequence: ACGCGCTGACGACCCGCTT
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Promoter: CMV/TO
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA