pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
(Plasmid #52505)

• Purpose: mammalian expression vector of 53BP1 28A mutant
• Depositing Lab: Daniel Durocher
• Publication: Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.

Backbone

• Vector backbone: pDEST 3' Triple Flag pcDNA5 FRT TO
• Backbone size w/o insert (bp): 6856
• Total vector size (bp): 12772
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1 28A
• Alt name: siRNA resistant and ATM site-deficient construct of 53BP1
• Species: H. sapiens (human)
• Mutation: All 28 SQ/TQ motifs in N-term of 53BP1 have been mutated to Ala; siRNA resistant mutation in 53BP1 is ACGCGCTGACGACCCGCTT
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • 52505.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA