pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1
(Plasmid #52504)

• Purpose: mammalian expression vector of BRCA1 wildtype siRNA resistant
• Depositing Lab: Daniel Durocher
• Publication: Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.

Backbone

• Vector backbone: pDEST 3' Triple Flag pcDNA5 FRT TO
• Backbone size w/o insert (bp): 6856
• Total vector size (bp): 8720
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Mutation: siRNA resistant sequence: AGTATAATC
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pDEST-FRT-TO-3xFLAG-BRCA1.gbk
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA