pRVdG-4BFP2-5postmGRASP
(Plasmid
#52498)
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PurposeExpresses mTagBFP2 and postsynaptic mGRASP component
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecSPBN (pSAD)
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Backbone manufacturerKarl-Klaus Conzelmann & Matthias Schnell
- Backbone size w/o insert (bp) 13854
- Total vector size (bp) 16017
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Vector typeMammalian Expression ; Rabies Virus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha at 37C
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemTagBFP2
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SpeciesSynthetic
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Insert Size (bp)705
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namepostsynaptic mGRASP
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Alt namepostmGRASP
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SpeciesSynthetic
- Promoter CMV
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVdG-4BFP2-5postmGRASP was a gift from Ian Wickersham (Addgene plasmid # 52498 ; http://n2t.net/addgene:52498 ; RRID:Addgene_52498)