pRVdG-4RFP
(Plasmid
#52489)
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PurposeExpresses TagRFP-T
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecSPBN (pSAD)
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Backbone manufacturerKarl-Klaus Conzelmann & Matthias Schnell
- Backbone size w/o insert (bp) 13782
- Total vector size (bp) 14517
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Vector typeRabies Virus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTagRFP-T
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SpeciesSynthetic
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Insert Size (bp)735
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVdG-4RFP was a gift from Sebastian Seung (Addgene plasmid # 52489 ; http://n2t.net/addgene:52489 ; RRID:Addgene_52489) -
For your References section:
Young dentate granule cells mediate pattern separation, whereas old granule cells facilitate pattern completion. Nakashiba T, Cushman JD, Pelkey KA, Renaudineau S, Buhl DL, McHugh TJ, Rodriguez Barrera V, Chittajallu R, Iwamoto KS, McBain CJ, Fanselow MS, Tonegawa S. Cell. 2012 Mar 30;149(1):188-201. doi: 10.1016/j.cell.2012.01.046. Epub 2012 Feb 23. 10.1016/j.cell.2012.01.046 PubMed 22365813