pRVdG-4mCherry
(Plasmid
#52488)
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PurposeExpresses mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecSPBN (pSAD)
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Backbone manufacturerKarl-Klaus Conzelmann & Matthias Schnell
- Backbone size w/o insert (bp) 13783
- Total vector size (bp) 14497
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Vector typeRabies Virus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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Alt namemC
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SpeciesSynthetic
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Insert Size (bp)714
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVdG-4mCherry was a gift from Sebastian Seung (Addgene plasmid # 52488 ; http://n2t.net/addgene:52488 ; RRID:Addgene_52488) -
For your References section:
Transgenic targeting of recombinant rabies virus reveals monosynaptic connectivity of specific neurons. Weible AP, Schwarcz L, Wickersham IR, Deblander L, Wu H, Callaway EM, Seung HS, Kentros CG. J Neurosci. 2010 Dec 8;30(49):16509-13. doi: 10.1523/JNEUROSCI.2442-10.2010. 10.1523/JNEUROSCI.2442-10.2010 PubMed 21147990