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Addgene

cSPBN-4GFP
(Plasmid #52487)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 52487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    cSPBN (pSAD)
  • Backbone manufacturer
    Karl-Klaus Conzelmann & Matthias Schnell
  • Backbone size w/o insert (bp) 13782
  • Total vector size (bp) 14502
  • Vector type
    Rabies Virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    Enhanced Green Fluorescent Protein
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative plasmid name: pRVdG-4EGFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cSPBN-4GFP was a gift from Sebastian Seung (Addgene plasmid # 52487 ; http://n2t.net/addgene:52487 ; RRID:Addgene_52487)

  • For your References section:

    Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons. Wickersham IR, Sullivan HA, Seung HS. Nat Protoc. 2010 Mar;5(3):595-606. doi: 10.1038/nprot.2009.248. Epub 2010 Mar 4. 10.1038/nprot.2009.248 PubMed 20203674
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