Human HMG-CoA lyase like 1 (HMGCLL1)
(Plasmid
#52434)
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Purposemammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHMGCLL1
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Alt nameHMG CoA lyase like 1
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SpeciesH. sapiens (human)
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Entrez GeneHMGCLL1 (a.k.a. RP11-418P12.1, bA418P12.1, er-cHL)
- Promoter CMV
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Tag
/ Fusion Protein
- HIS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human HMG-CoA lyase like 1 (HMGCLL1) was a gift from Henry Miziorko (Addgene plasmid # 52434 ; http://n2t.net/addgene:52434 ; RRID:Addgene_52434) -
For your References section:
Identification and characterization of an extramitochondrial human 3-hydroxy-3-methylglutaryl-CoA lyase. Montgomery C, Pei Z, Watkins PA, Miziorko HM. J Biol Chem. 2012 Sep 28;287(40):33227-36. Epub 2012 Aug 3. 10.1074/jbc.M112.393231 PubMed 22865860