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Addgene

Human HMG-CoA lyase (HL)
(Plasmid #52433)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMG CoA lyase
  • Alt name
    HMGCL
  • Species
    H. sapiens (human)
  • Entrez Gene
    HMGCL (a.k.a. HL)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • S (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes the mature mitochondrial form of human HMGCL.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human HMG-CoA lyase (HL) was a gift from Henry Miziorko (Addgene plasmid # 52433 ; http://n2t.net/addgene:52433 ; RRID:Addgene_52433)

  • For your References section:

    Influence of multiple cysteines on human 3-hydroxy-3-methylglutaryl-CoA lyase activity and formation of inter-subunit adducts. Montgomery C, Miziorko HM. Arch Biochem Biophys. 2011 Jul;511(1-2):48-55. doi: 10.1016/j.abb.2011.04.004. Epub 2011 Apr 13. 10.1016/j.abb.2011.04.004 PubMed 21514269
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