p21-Reporter
(Plasmid #52432)

• Purpose: DNA damage inducible Red Fluorescent Protein for mammalian expression
• Depositing Lab: Joel Voldman
• Publication: Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. doi: 10.1021/ac501412c. Epub 2014 Jul 7.

Backbone

• Vector backbone: pTurboRFP-PRL
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 4127
• Total vector size (bp): 4794
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p21-p53 Tandem Response Element Promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 667
• Promoter: 13X repeated p21-p53 response element
• Tag / Fusion Protein:
  • TurboRFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SV40pro_F_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Vector backbone was purchased from Evrogen and the insert was cloned by Blue Heron.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry