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Addgene

Human Mevalonate diphosphate decarboxylase (MDD)
(Plasmid #52431)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23d(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mevalonate diphosphate decarboxylase
  • Alt name
    MDD
  • Species
    H. sapiens (human)
  • Entrez Gene
    MVD (a.k.a. FP17780, MPD)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 5’ primer (5’-TTCCCCATGGCCTCGGAGAAGC-3’) encoded an NcoI site harboring the ATG start codon; the 3’ primer (5’-GAGGCTCGAGGGCAGCTGGCTTCGGCAGGC-3’) encoded a XhoI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Mevalonate diphosphate decarboxylase (MDD) was a gift from Henry Miziorko (Addgene plasmid # 52431 ; http://n2t.net/addgene:52431 ; RRID:Addgene_52431)

  • For your References section:

    Human mevalonate diphosphate decarboxylase: characterization, investigation of the mevalonate diphosphate binding site, and crystal structure. Voynova NE, Fu Z, Battaile KP, Herdendorf TJ, Kim JJ, Miziorko HM. Arch Biochem Biophys. 2008 Dec 1;480(1):58-67. doi: 10.1016/j.abb.2008.08.024. Epub 2008 Sep 18. 10.1016/j.abb.2008.08.024 PubMed 18823933
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