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Addgene

Human Phosphomevalonate kinase (PMK)
(Plasmid #52430)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Phosphomevalonate kinase
  • Alt name
    PMK
  • Species
    H. sapiens (human)
  • Entrez Gene
    PMVK (a.k.a. HUMPMKI, PMK, PMKA, PMKASE, POROK1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Phosphomevalonate kinase (PMK) was a gift from Henry Miziorko (Addgene plasmid # 52430 ; http://n2t.net/addgene:52430 ; RRID:Addgene_52430)

  • For your References section:

    Phosphomevalonate kinase: functional investigation of the recombinant human enzyme. Herdendorf TJ, Miziorko HM. Biochemistry. 2006 Mar 14;45(10):3235-42. 10.1021/bi052231u PubMed 16519518
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