Human Mevalonate Kinase (MK)
(Plasmid #52429)

• Purpose: bacterial expression of human Mevalonate Kinase (MK)
• Depositing Lab: Henry Miziorko
• Publication: Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-54.

Backbone

• Vector backbone: pET-3d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4637
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Human Mevalonate Kinase
• Alt name: MK
• Species: H. sapiens (human)
• Mutation: see comments
• Entrez Gene: MVK (a.k.a. LRBP, MK, MVLK, POROK3)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

During PCR amplification, the codon for leucine 2 (TTG) was changed to valine (GTG). This single base mutation created an NcoI site (CCATGG) that overlapped the start codon of mevalonate kinase.