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Addgene

pECE-HA-FOXO3 R248K R250K
(Plasmid #52427)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FOXO3a
  • Alt name
    FKHRL1
  • Alt name
    forkhead family
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2038
  • Mutation
    R248K R250K, mutant methylation sites
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generated from Addgene plasmid #1787, HA-FOXO3a WT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECE-HA-FOXO3 R248K R250K was a gift from Steven Weinman (Addgene plasmid # 52427 ; http://n2t.net/addgene:52427 ; RRID:Addgene_52427)

  • For your References section:

    Regulation of FOXO3 by phosphorylation and methylation in hepatitis C virus infection and alcohol exposure. Tikhanovich I, Kuravi S, Campbell RV, Kharbanda KK, Artigues A, Villar MT, Weinman SA. Hepatology. 2014 Jan;59(1):58-70. doi: 10.1002/hep.26618. Epub 2013 Nov 20. 10.1002/hep.26618 PubMed 23857333
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