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Addgene

pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
(Plasmid #52421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-(-)-Hyg
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5508
  • Total vector size (bp) 7089
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion NLS-EGFP-P2A-Cherry-CAAX
  • Species
    Synthetic
  • Insert Size (bp)
    1581
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pcDNA3_FOR
  • 3′ sequencing primer pcDNA3_REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX was a gift from Bertrand Collet (Addgene plasmid # 52421 ; http://n2t.net/addgene:52421 ; RRID:Addgene_52421)
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