pTRE2-Hyg-omMx1
(Plasmid #52420)

• Purpose: Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein
• Depositing Lab: Bertrand Collet
• Publication: Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8. doi: 10.1016/j.jviromet.2012.01.014. Epub 2012 Mar 1.

Backbone

• Vector backbone: pTRE2-Hyg
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5298
• Total vector size (bp): 7164
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
• Species: Oncorhynchus mykiss
• Insert Size (bp): 1866
• GenBank ID: NM_001171901
• Promoter: TRE2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TRE2F
• 3′ sequencing primer: TRE2R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control sequencing has found two amino acid residue substitutions, G431E and M577I, compared to the depositor's full sequence. These differences are believed to be natural inter-population polymorphisms and are not thought to affect plasmid function.