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Addgene

pJAF215
(Plasmid #52402)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFLAG-CMV-14
  • Backbone manufacturer
    Sigma
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arf1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Arf1
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAF215 was a gift from Gregory Pazour (Addgene plasmid # 52402 ; http://n2t.net/addgene:52402 ; RRID:Addgene_52402)

  • For your References section:

    Arf4 is required for Mammalian development but dispensable for ciliary assembly. Follit JA, San Agustin JT, Jonassen JA, Huang T, Rivera-Perez JA, Tremblay KD, Pazour GJ. PLoS Genet. 2014 Feb 20;10(2):e1004170. doi: 10.1371/journal.pgen.1004170. eCollection 2014 Feb. PubMed 24586199
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