pJAF215
(Plasmid
#52402)
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PurposeExpresses Flag-tagged mouse Arf1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3XFLAG-CMV-14
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Backbone manufacturerSigma
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArf1
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJAF215 was a gift from Gregory Pazour (Addgene plasmid # 52402 ; http://n2t.net/addgene:52402 ; RRID:Addgene_52402) -
For your References section:
Arf4 is required for Mammalian development but dispensable for ciliary assembly. Follit JA, San Agustin JT, Jonassen JA, Huang T, Rivera-Perez JA, Tremblay KD, Pazour GJ. PLoS Genet. 2014 Feb 20;10(2):e1004170. doi: 10.1371/journal.pgen.1004170. eCollection 2014 Feb. PubMed 24586199