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UASp-Fz4
(Plasmid #52400)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASP
  • Backbone size w/o insert (bp) 9939
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila Frizzled 4
  • Alt name
    Fz4
  • Alt name
    Dfz4
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3500
  • Entrez Gene
    fz4 (a.k.a. Dmel_CG4626, CG4626, DFz4, Dfz4, Dm Fz4, Dmel\CG4626, Fz4, anon-WO0170980.10, anon-WO0170980.11)
  • Promoter UAS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer pUASp-5
  • 3′ sequencing primer pUASp-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UASp-Fz4 was a gift from Roel Nusse (Addgene plasmid # 52400 ; http://n2t.net/addgene:52400 ; RRID:Addgene_52400)

  • For your References section:

    A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration. McElwain MA, Ko DC, Gordon MD, Fyrst H, Saba JD, Nusse R. PLoS One. 2011;6(11):e26993. doi: 10.1371/journal.pone.0026993. Epub 2011 Nov 1. 10.1371/journal.pone.0026993 PubMed 22069480
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