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PurposeThe plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLXSN
- Backbone size w/o insert (bp) 5874
- Total vector size (bp) 6700
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHPV16 E6E7
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Insert Size (bp)770
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GenBank IDNP_041325.1 NP_041326.1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI, (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pLXSNF
- 3′ sequencing primer pLXSNR (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLXSN16E6E7 was a gift from Denise Galloway (Addgene plasmid # 52394 ; http://n2t.net/addgene:52394 ; RRID:Addgene_52394) -
For your References section:
The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. Halbert CL, Demers GW, Galloway DA. J Virol. 1991 Jan;65(1):473-8. PubMed 1845902
Map uploaded by the depositor.
