5' MBD3 TALEN
(Plasmid
#52358)
-
PurposeTALEN vector for targeting human MBD3 locus (5')
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 52358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepc-GoldyTALEN
-
Backbone manufacturerDan Carlson lab plasmids (from addgene)
-
Vector typeTALEN
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNI NN NN NN NN HD HD NI NN NG NG NN NG NN NN NN NN NG NN
- Promoter CAGGS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (not destroyed)
- 3′ cloning site Esp3I (not destroyed)
- 5′ sequencing primer AGGCGACACACGAAGACATC
- 3′ sequencing primer CGGCGATTGACTCTTCTGAT (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
5' MBD3 TALEN was a gift from Jacob Hanna (Addgene plasmid # 52358 ; http://n2t.net/addgene:52358 ; RRID:Addgene_52358) -
For your References section:
Deterministic direct reprogramming of somatic cells to pluripotency. Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA, Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z, Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern N, Hanna JH. Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 2013 Sep 18. 10.1038/nature12587 PubMed 24048479