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Addgene

pEL333
(Plasmid #52349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-3xFLAG
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6299
  • Total vector size (bp) 7472
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    swan-1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1167
  • Entrez Gene
    swan-1 (a.k.a. CELE_F53C11.8)
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEL333 was a gift from Erik Lundquist (Addgene plasmid # 52349 ; http://n2t.net/addgene:52349 ; RRID:Addgene_52349)

  • For your References section:

    SWAN-1, a Caenorhabditis elegans WD repeat protein of the AN11 family, is a negative regulator of Rac GTPase function. Yang Y, Lu J, Rovnak J, Quackenbush SL, Lundquist EA. Genetics. 2006 Dec;174(4):1917-32. Epub 2006 Sep 15. 10.1534/genetics.106.063115 PubMed 16980389
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