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pCCL-ReDNAP (Y427A)
(Plasmid #52346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CEN6-ARS4/ColE1 Yeast/Bacteria Shuttle Vector
  • Backbone size w/o insert (bp) 6105
  • Total vector size (bp) 9069
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    HIS4

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Recoded TP-DNAP
  • Alt name
    ReDNAP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2964
  • Promoter REV1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTCTAAAGACCTGGTTCTTAAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCL-ReDNAP (Y427A) was a gift from Chang Liu (Addgene plasmid # 52346 ; http://n2t.net/addgene:52346 ; RRID:Addgene_52346)

  • For your References section:

    An orthogonal DNA replication system in yeast. Ravikumar A, Arrieta A, Liu CC. Nat Chem Biol. 2014 Mar;10(3):175-7. doi: 10.1038/nchembio.1439. Epub 2014 Feb 2. 10.1038/nchembio.1439 PubMed 24487693
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