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Addgene

AAVS1-CAG-hrGFP
(Plasmid #52344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1-CAG
  • Backbone size w/o insert (bp) 8085
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hrGFP
  • Alt name
    Vitality hrGFP II
  • Species
    Synthetic
  • Insert Size (bp)
    724
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer pCAX-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid is NOT the inducible AAVS1-TRE3G-EGFP construct described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-CAG-hrGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52344 ; http://n2t.net/addgene:52344 ; RRID:Addgene_52344)

  • For your References section:

    A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, Sauvey C, Du Z, Zhang SC. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. 10.1002/stem.1653 PubMed 24497442
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