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Addgene

hAAVS1 TALEN Left
(Plasmid #52341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JDS78
  • Backbone manufacturer
    Keith Joung lab (Addgene Plasmid # 32290)
  • Backbone size w/o insert (bp) 6447
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAVS1 TALEN Left
  • Species
    Synthetic
  • Insert Size (bp)
    1640
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer GGAGTAACAGCGGTAGAGG
  • 3′ sequencing primer GCGGGATCGGGCCTGGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results identified a partial duplication within the TALEN repeat region of this plasmid relative to the expected sequence. The depositing lab confirmed that the plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hAAVS1 TALEN Left was a gift from Su-Chun Zhang (Addgene plasmid # 52341 ; http://n2t.net/addgene:52341 ; RRID:Addgene_52341)

  • For your References section:

    A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, Sauvey C, Du Z, Zhang SC. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. 10.1002/stem.1653 PubMed 24497442
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