PR594-His-bio
(Plasmid #52332)

• Purpose: Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
• Depositing Lab: Gavin Wright
• Publication: Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6749
• Total vector size (bp): 7001
• Modifications to backbone: His tag, linker, enzymatic biotinylation sequence, and rat CD4 d3+4 (HisLBio-CD4d3+4).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PR594
• Alt name: Neuron-specific protein family member 1
• Alt name: NSG1
• Species: H. sapiens (human)
• Insert Size (bp): 252
• Entrez Gene: NSG1 (a.k.a. D4S234, D4S234E, NEEP21, P21)
• Promoter: CMV
• Tags / Fusion Proteins:
  • His tag (N terminal on backbone)
  • enzymatic biotinylation sequence (N terminal on backbone)
  • rat CD4 d3+4 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was chemically synthesised.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Article ref 110