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Addgene

pFGL889
(Plasmid #52321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL815N
  • Backbone size (bp) 6869
  • Modifications to backbone
    1. A truncated ILV2SUR (2.3kb, without the first exon) was introduced in between Xho1 and EcoR1 sites. 2. A 1-kb 3' downstream region of ILV2 was inserted between Sph1 and Hind3 sites.
  • Vector type
    Bacterial Expression ; Agrobacterium
  • Selectable markers
    Sulfonylurea (Chlorimuron ethyl)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL889 was a gift from Naweed Naqvi (Addgene plasmid # 52321 ; http://n2t.net/addgene:52321 ; RRID:Addgene_52321)

  • For your References section:

    Sulfonylurea resistance reconstitution as a novel strategy for ILV2-specific integration in Magnaporthe oryzae. Yang F, Naqvi NI. Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 10.1016/j.fgb.2014.04.005. 10.1016/j.fgb.2014.04.005 PubMed 24769367
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