pET-Trx-mSA2
(Plasmid #52320)

• Purpose: Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
• Depositing Lab: Sheldon Park
• Publication: Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 10.1007/s00253-014-5682-y. Epub 2014 Apr 2.

Backbone

• Vector backbone: pET-MBP
• Total vector size (bp): 6289
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: monomeric streptavidin 2
• Alt name: mSA2
• Alt name: mSA2.2
• Species: Synthetic
• Insert Size (bp): 357
• Mutation: S25H mutation compared to streptavidin
• Promoter: T7
• Tags / Fusion Proteins:
  • Thioredoxin (N terminal on backbone)
  • His (N terminal on backbone)
  • S tag (N terminal on backbone)
  • TEV site (N terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry