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PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-MBP
- Total vector size (bp) 6886
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemonomeric streptavidin 2
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Alt namemSA2
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Alt namemSA2.2
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SpeciesSynthetic
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Insert Size (bp)357
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MutationS25H mutation compared to streptavidin
- Promoter T7
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- MBP (N terminal on backbone)
- TEV (N terminal on insert)
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-MBP-mSA2 was a gift from Sheldon Park (Addgene plasmid # 52319 ; http://n2t.net/addgene:52319 ; RRID:Addgene_52319) -
For your References section:
Expression and purification of soluble monomeric streptavidin in Escherichia coli. Demonte D, Dundas CM, Park S. Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 10.1007/s00253-014-5682-y. Epub 2014 Apr 2. 10.1007/s00253-014-5682-y PubMed 24691867