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Addgene

pET-MBP-mSA2
(Plasmid #52319)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52319 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-MBP
  • Total vector size (bp) 6886
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric streptavidin 2
  • Alt name
    mSA2
  • Alt name
    mSA2.2
  • Species
    Synthetic
  • Insert Size (bp)
    357
  • Mutation
    S25H mutation compared to streptavidin
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • MBP (N terminal on backbone)
    • TEV (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-MBP-mSA2 was a gift from Sheldon Park (Addgene plasmid # 52319 ; http://n2t.net/addgene:52319 ; RRID:Addgene_52319)
  • For your References section:

    Expression and purification of soluble monomeric streptavidin in Escherichia coli. Demonte D, Dundas CM, Park S. Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 10.1007/s00253-014-5682-y. Epub 2014 Apr 2. 10.1007/s00253-014-5682-y PubMed 24691867