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Addgene

His Puc (aa 1-393)
(Plasmid #52295)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Puc
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    puc (a.k.a. Dmel_CG7850, 0238/03, 1351/08, CG7850, Dmel\CG7850, JNK-DSP, PUC, PUC/MKP, Puc, hrt, l(3)84Eh, l(3)A251.1, l(3)j4E1, vco)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer LacI-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When compared to GenBank reference sequence NP_524273.1, the Puc insert in this plasmid contains an additional 3 amino acids between aa 309 & 310. The numbering of Puc ending at aa 393 does not count the starting Met but does include the additional 3 amino acids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His Puc (aa 1-393) was a gift from Brenda Schulman (Addgene plasmid # 52295 ; http://n2t.net/addgene:52295 ; RRID:Addgene_52295)

  • For your References section:

    Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA. Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022. 10.1016/j.molcel.2009.09.022 PubMed 19818708
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