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His-MBP human SPOP (aa 28-337)
(Plasmid #52294)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPOP
  • Alt name
    SPOP aa 28-337
  • Alt name
    SPOP MATH-BTB+
  • Species
    H. sapiens (human)
  • Entrez Gene
    SPOP (a.k.a. BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His-MBP (N terminal on insert)
    • TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer LacI-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SPOP-MATH-BTB+ were fused to the C terminus of His-tagged maltose-binding protein (His-MBP) and subcloned into the first cloning site of pRSFDuet vector (Novagen). TEV cleavage sites were engineered between the His-MBP tag and the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-MBP human SPOP (aa 28-337) was a gift from Brenda Schulman (Addgene plasmid # 52294 ; http://n2t.net/addgene:52294 ; RRID:Addgene_52294)

  • For your References section:

    Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA. Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022. 10.1016/j.molcel.2009.09.022 PubMed 19818708
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