pGEX-TEVsite-human Glomulin
(Plasmid
#52292)
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Purposebacterial expression of human Glomulin
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Modifications to backbonemodified to contain a TEV proteolytic cleavage site
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGlomulin
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SpeciesH. sapiens (human)
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Entrez GeneGLMN (a.k.a. FAP, FAP48, FAP68, FKBPAP, GLML, GVM, VMGLOM)
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- TEV (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-TEVsite-human Glomulin was a gift from Brenda Schulman (Addgene plasmid # 52292 ; http://n2t.net/addgene:52292 ; RRID:Addgene_52292) -
For your References section:
Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface. Duda DM, Olszewski JL, Tron AE, Hammel M, Lambert LJ, Waddell MB, Mittag T, DeCaprio JA, Schulman BA. Mol Cell. 2012 Aug 10;47(3):371-82. doi: 10.1016/j.molcel.2012.05.044. Epub 2012 Jun 28. 10.1016/j.molcel.2012.05.044 PubMed 22748924