pBacPak-FLAG-HIS-Sgf11
(Plasmid
#52287)
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PurposeExpresses Drosophila Sgf11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBacPak
- Total vector size (bp) 6159
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSgf11
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Alt nameATXN7L3
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Alt nameCG13379
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SpeciesD. melanogaster (fly)
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Insert Size (bp)591
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Entrez GeneSgf11 (a.k.a. Dmel_CG13379, CG13379, Dmel\CG13379, dSgf11)
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Tag
/ Fusion Protein
- HIS, FLAG (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBacPak-FLAG-HIS-Sgf11 was a gift from Jerry Workman (Addgene plasmid # 52287 ; http://n2t.net/addgene:52287 ; RRID:Addgene_52287) -
For your References section:
Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration. Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, Washburn MP, Workman JL, Abmayr SM. Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113. 10.1101/gad.225151.113 PubMed 24493646